A two-stage poly(ethylenimine)-mediated cytotoxicity: implications for gene transfer/therapy
نویسندگان
چکیده
منابع مشابه
Polyethylenimine-mediated gene delivery to the lung and therapeutic applications
Nonviral gene delivery is now considered a promising alternative to viral vectors. Among nonviral gene delivery agents, polyethylenimine (PEI) has emerged as a potent candidate for gene delivery to the lung. PEI has some advantages over other polycations in that it combines strong DNA compaction capacity with an intrinsic endosomolytic activity. However, intracellular (mainly the nuclear membra...
متن کاملPolyethylenimine-mediated cochlear gene transfer in guinea pigs.
OBJECTIVE To demonstrate and compare polycationic-mediated cochlear gene transfer with linear polyethylenimine (PEI) via cochleostomy and osmotic pump infusion method. DESIGN A dissociated cochlear culture was used to select the optimum nitrogen to phosphate ratio of PEI/DNA complexes to be used in vivo. The PEI-enhanced green fluorescent protein reporter gene DNA complex was introduced with ...
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UNLABELLED The clinical application of gene silencing mediated by small interfering RNA (siRNA) has been limited by the lack of efficient and safe carriers. Phospholipid modification of low molecular weight polyethylenimine (PEI 1.8 kDa) dramatically increased its gene down-regulation capacity while keeping cytotoxicity levels low. The silencing efficacy was highly dependent on the nature of th...
متن کاملCross-linked polyethylenimine as potential DNA vector for gene delivery with high efficiency and low cytotoxicity.
Polyethylenimine (PEI) has been known as an efficient gene carrier with the highest cationic charge potential. High transfection efficiency of PEI, along with its cytotoxicity, strongly depends on its molecular weight. To enhance its gene delivery efficiency and minimize cytotoxicity, we have synthesized small cross-linked PEI with biodegradable linkages and evaluated their transfection efficie...
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In this paper, we present a two-stage model for ranking of decision making units (DMUs) using interval analytic hierarchy process (AHP). Since the efficiency score of unity is assigned to the efficient units, we evaluate the efficiency of each DMU by basic DEA models and calculate the weights of the criteria using proposed model. In the first stage, the proposed model evaluates decision making...
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ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2005
ISSN: 1525-0016
DOI: 10.1016/j.ymthe.2005.02.010